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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in several tumors, which results in chromosome instability, and therefore could add to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital function in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be described. Two additional splice variants ended up described but comprehensive nucleotide sequence information just isn't but readily available.

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Blocking peptides are peptides that bind particularly into the target antibody and block antibody binding. These peptide usually contains the epitope regarded via the antibody.

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This guidebook outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

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This guidebook outlines further more possible optimization of cellular and immunoassay parameters to make certain the very best results are...

Antibodies bound to the blocking peptide no longer bind towards check here the epitope within the focus on protein. By evaluating the staining in the blocked antibody versus the antibody on your own, you can see which staining is specific.

Target Information Cyclin E belongs to the highly conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which add towards the temporal coordination of each mitotic celebration. Cyclin E types a posh with and functions for a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress by S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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